In a programme first, all five of the Chasers - The Barrister, The Beast, The Governess, The Sinnerman and The Vixen - will take part in the milestone episode, airing on ITV on Thursday, 29 September.
During the hour-long special, each contestant will face one of four different Chasers during the 'Cash Builder' round, while a fifth Chaser will play the Final Chase round.
The Chase, which was voted Best Daytime Show at the National Television Awards, has been running for seven years now and more than three million people watch it every day.
Bradley Walsh, who hosts the show, said: "We've made it to the 1000th episode of The Chase as the show is so entertaining and informative. I'm so lucky I get to be a part of such a great team and have a laugh at work, I even learn some things too. People love it because they can see how much we are enjoying ourselves. The special is going to be one Chase fans won't want to miss."
The 1000th episode of The Chase airs Thursday 29th September at 5pm on ITV