There's no denying we're a nation of dog lovers! It's the second time a talented canine has beat off all human competition, after Ashleigh Butler and her dog Pudsey won the show in 2012.
After four and a half million votes were cast, it was revealed the prancing pup had beat off the strong competition from Welsh choir Cor Glanaethwy, who came third, and magician Jamie Raven, who came second thanks to his insane magic trick with a lemon. Seriously, how did he do that?!
The winning performance featured Matisse escaping from 'police officer' Jules by walking across a tightrope suspended high above the stage, which was pretty nerve wracking to watch!
Even the judges were totally won over by the border collie, with David Walliams announcing he was no longer a "cat person" but a "dog person".
The duo will perform at the Royal Variety Performance, and also take home a cash prize of £250,000. So we expect to see Matisse is a shiny new collar come performance night!
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Watch their winning performance below: