Nick found fame as a squeaky clean teenager alongside his siblings Joe and Kevin in the Jonas Brothers, and the band were famed for wearing purity rings.
However, recently Nick's ring - which he wore to signify his virginity - was noted to have disappeared from his finger.
Yesterday, the 22-year-old singer appeared on The Wendy Williams Show and cleared up the mystery, telling host Wendy that he's an adult "in all ways".
"Am I still a virgin?" Nick said, when asked the direct question. "No, no. As I said, I'm an adult in all ways and comfortable with who I am."
Nick is currently dating Olivia Culpo, a former Miss Universe, and has been romantically linked to Gigi Hadid and Delta Goodrem in the past.
Looking back on his younger years, Nick confirmed to the chat show host that he has no regrets about wearing a purity ring.
He said: "I don't regret it. I think it was an important part of my childhood and it was that.
"I think as I grew up, I sort of figured out what was important to me and my own belief system. We all grow up and we all live life and find out what's important to us - that's what happened.
"I went on that journey myself and I'm now comfortable with who I am and what I believe in and I'm an adult in all ways."
Nick, Joe and Kevin formed the Jonas Brothers in 2005 and, along with their pop career, starred in Disney movies Camp Rock and Camp Rock 2: The Final Jam.
In October last year, the siblings announced the Jonas Brothers were splitting after eight years together.
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