The two singers set pulses racing on Saturday (19 July) as they donned leather-like jumpsuits while on set in Los Angeles.
Rita and Iggy, who wore contrasting outfits, were joined by Kill Bill actor Michael Madsen.
'Shine Ya Light' singer Rita showed off her cleavage in a plunging zip-up red suit, while Iggy - who sported dip dyed hair - wore a similar white and black bodysuit.
The pair were later seen sharing a giggle as they walked together in white dressing gowns.
'Black Widow' is rumoured to have been inspired by Quentin Tarantino's Kill Bill movie, so perhaps that's why Michael joined them on set?!
Earlier this month, Iggy shared the artwork for the single, and it definitely looks like some martial arts is involved!
We can't wait to see the video!
Of course, fans were treated to a performance of 'Black Widow' for the very first time earlier this month, when Rita joined Iggy on stage during her set at London's Wireless Festival.
'Black Widow' is taken from Iggy's newly-released album, The New Classic. The collaborative track is catchy, punchy and sure to be another hit for the girls!
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Watch Iggy and Rita perform 'Black Widow' at Wireless below: