The comedian referenced the now-infamous leaked CCTV footage showing Solange attacking her brother-in-law Jay Z in a lift following the Met Ball in New York earlier this month.
Advising the nominees at Theatre Royal, Drury Lane on what happens if they don't win, Graham quipped: "If you do get beaten, at least it wasn't in a lift by your wife's sister."
(Last week, Bey, Jay and Solange issued a statement on the video footage, saying: "Jay and Solange each assume their share of responsibility for what has occurred. They both acknowledge their role in this private matter that has played out in the public. They both have apologised to each other and we have moved forward as a united family.")
Beyoncé, Jay and Solange weren't the only ones who Graham took aim at - Gary Barlow, Good Morning Britain and Simon Cowell also got a bit of a tongue lashing.
While discussing Gary's decision to leave X Factor, Graham managed to throw in a sneaky reference to those recent tax allegations surrounding the Take That star. "That's the thing with Gary," said Graham. "He's not very good with returns."
After a collective gasp from the audience, Graham added: "Even Gary is watching this going, 'Actually, that's quite funny.'"
On the subject of people leaving TV shows, Graham went on to mention Sir Bruce Forsyth, who has quit Strictly Come Dancing and, ouch, "all the viewers who have left Good Morning Britain."
The camera then cut to Susanna Reid shaking her head but stifling a laugh.
Meanwhile, music mogul Simon Cowell was also at the receiving end of a joke at the expense of X Factor-themed musical I Can't Sing, which closed after just two months.
Noting it was a shame Simon couldn't make the BAFTAs, Graham said: "After his recent West End musical, it would be nice for him to see what a full theatre looks like."
Oh, naughty Graham...
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BAFTAs 2014 red carpet in pictures:
Copyright: WENN Daniel Deme