The Hollywood couple, who are proud parents to six-month old daughter Everly, met on the set of the 2006 dance film.
Channing and Jenna showed off their moves at the Ocean Drive Magazine anniversary party, shortly after Witches Of East End actress Jenna walked the red carpet in a daring white dress at the Miami bash.
Once the party kicked off, all eyes were on the loved-up couple who took centre stage on the dance floor.
Recreating their romantic moments, Channing wrapped his arms around Jenna and later picked up his wife and twirled her around in front of onlookers.
Channing, 33, also danced for Jenna when the DJ played Ginuwine's 'Pony', recreating his famous moves from his 2012 stripper movie Magic Mike.
The couple danced to hits by Janet Jackson, Justin Timberlake, Jay Z and Rihanna. Real-life dancer Jenna tweeted after the event: "My requests: 90's music + Janet! Can't go wrong!"
Step Up told the story of the disadvantaged Tyler Gage (Channing), who receives the opportunity of a lifetime to earn a scholarship and dance with an up and coming privileged dancer, Nora Clark (Jenna).
Channing and Jenna's chemistry continued off-screen and subsequently the couple tied the knot in a romantic ceremony in Malibu in 2009.
In December 2012, the pair revealed that they were expecting their first baby together, and on 31 May last year, Channing and Jenna welcomed daughter Everly in London where Channing was shooting new movie Jupiter Ascending at the time.
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