The British model, who turns 40 in January, will strip for a pictorial to be published in the iconic men's magazine's January/February 2013 edition, it has been confirmed.
Playboy founder Hugh Hefner told the Los Angeles Times that Kate was a "natural fit" for the publication, adding: "She's a worldwide celebrity and iconic and crosses the boundaries from sexual imagery to upscale modelling."
The magazine's editorial director Jimmy Jellinek added he wanted a global icon to mark the global brand's anniversary, saying: "She's the perfect partner for us to help launch the next 60 years."
Hugh's son Cooper Hefner added on Twitter: "Kate Moss will be gracing the cover of our 60th anniversary issue this January. It'll be a great issue."
Croydon-born Kate's pictorial has been shot by Marcus Pigott and Mert Atlas, and will be on sale from December 2013. Jimmy has promised fans will see her posing in the traditional Playboy attire of "bunny ears, French cuffs, silk stockings and a come-hither smile."
Hugh Hefner founded Playboy magazine in Chicago in 1953 using $1,000 that he borrowed from his mother. The very first nude centerfold was Marilyn Monroe.