The TOWIE hunk yelled "in your face, Lucy" when he accepted the prize, voted for by the British public, during Thursday night's episode of the Channel 5 show.
Mario and the rest of the remaining housemates - Vicky Entwistle, Charlotte Crosby, Abz Love, Lauren Harries and Carol McGiffin - were gathered together for their own glitzy awards show ahead of Friday night's finale..
Vicky presented Mario with his prize. Mario feigned shock and surprise, fanning his face, before taking to the mini stage for his acceptance speech.
"I want to thank mum and dad," he said. "I want to thank you all for bringing out the sexiest in me. Abz from 5ive, you are a sexy guy. Lauren, just working it. Vicky, just look at her. Hot! Carol, you're sex on legs, what can I say? And Charlotte. Thank you."
Then, when Mario took his seat afterwards, he shouted out: "In your face, Lucy!"
The Italian tailor split with his TOWIE co-star back in April. Viewers of the ITV2 show saw the bitter fallout from their break up on the last season, but by the time the finale rolled around it appeared they'd managed to put their differences aside.
Lucy hasn't directly responded to Mario but she did tweet last night: "Boring..." A reference to CBB? Or something else entirely?
Celebrity Big Brother's final airs tonight.