Harry, Niall Horan, Zayn Malik, Liam Payne and Louis Tomlinson of the British boy band collected their awards including Choice Music Group and Choice Love Song at the ceremony held in California.
But Harry, who also scooped two individual awards for Choice Hottie and Choice Smile, sent fans into a frenzy as he showed off his twerking skills.
The curly-haired heartthrob performed an impromptu dance when he was singled out by the night's hosts Pretty Little Liars' Lucy Hale and Glee's Darren Criss, while sitting next to Ed Sheeran.
The twerk, which lasted for no more than two seconds, sent Twitter into meltdown and the hashtag '#HarryDroppedThatThunThunThun' became a worldwide trend.
While Harry's moves of the popular dance craze may have impressed his legion of female fans, it doesn't look like it's a dance he'll be repeating anytime soon. He tweeted after the awards: "Note to self. Don't 'twerk'."
Ed, who laughed hysterically as Harry performed for the crowd, later took to Twitter to voice his opinion. The 'Give Me Love' singer, who also bagged an award for Best Breakthrough, said: "One more note, people need to stop encouraging little kids to twerk, it's odd."
Ed then attached a photo of Harry and said: "Top twerker 2k7."
Note to self. Don't 'twerk'.— Harry Styles (@Harry_Styles) August 12, 2013
One Direction, who have just wrapped up the US leg of their greulling 'Take Me Home' tour, also treated fans to a performance of their new single, 'Best Song Ever'.
Tweeting about their victory, Niall said: "Yessss ! Thank you guys so much for the awards. U mean so much to us! Wouldn't be doin it without you! Thank you America! Chill tonight!"
Watch a video of Harry twerking below:
More pictures from the Teen Choice Awards 2013 below:
Copyright: WENN Nikki Nelson