The blonde actress, who plays Penny in the hit sitcom, unveiled her shorter do via Instagram on Saturday night (31 May), saying: "Thank you @clsymonds for bringing out my inner Peter Pan :) #byronbeverlyhills #justcutitoff."
Kaley's hair was cut by stylist Christine Symonds at the Byron Williams Salon in Beverly Hills.
Christine shared an alternative picture of Kaley's new crop to her own Instagram, saying: "This little lamb went shorter. Love playing at the hair salon with @normancook. #shortandsassy #summercut #beauty #hot #babe #byronwilliamssalon."
Kaley, 28, previously cut inches off her hair in April, switching her flowing long locks for a shoulder-grazing bob.
What will be next?!
Last night, the starlet took her new hair for its first night out, joining husband Ryan Sweeting and friends at the Hollywood Bowl to watch Bruno Mars and Pharrell Williams in concert.
"Best night at the bowl!! #pharrell #brunomars #happy," she told fans.
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